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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1984-11-13
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pubmed:databankReference |
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pubmed:abstractText |
A method was devised to clone immunoglobulin VH-region genes located on selected restriction fragments from genomic DNA directly into M13 vectors for subsequent nucleotide sequence analysis. Ten recombinant M13 clones representing four so far unknown VH-region genes of the VNP-gene family have been analysed. Sequence comparison shows that these genes are closely related to other VH-genes of the VNP gene family. One of the VH-genes exhibits a so far unobserved unusual length of 100 2/3 codons and appears to be functional. Analysis of the variation of the isolated VH-genes suggests that framework and complementarity determining regions are exposed to separate types of selective pressures.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-103630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-111144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-115000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-498284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6101208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6154766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6222379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6285297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6287467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6308665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6315403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6329676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6403356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6420501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6432337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6765233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6765983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6766210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6769593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6774258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6787590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6788376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6789211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-6791833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-7188353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-7231520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-881736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091043-97089
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6887-900
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6091043-Animals,
pubmed-meshheading:6091043-Bacteriophage lambda,
pubmed-meshheading:6091043-Base Sequence,
pubmed-meshheading:6091043-Cloning, Molecular,
pubmed-meshheading:6091043-Coliphages,
pubmed-meshheading:6091043-DNA, Recombinant,
pubmed-meshheading:6091043-DNA Restriction Enzymes,
pubmed-meshheading:6091043-Escherichia coli,
pubmed-meshheading:6091043-Genes,
pubmed-meshheading:6091043-Hybridomas,
pubmed-meshheading:6091043-Immunoglobulin Heavy Chains,
pubmed-meshheading:6091043-Immunoglobulin Variable Region,
pubmed-meshheading:6091043-Mice,
pubmed-meshheading:6091043-Nucleic Acid Hybridization
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pubmed:year |
1984
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pubmed:articleTitle |
Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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