Linked Life Data

6088527

Source:http://linkedlifedata.com/resource/pubmed/id/6088527

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1984-10-5
pubmed:databankReference
pubmed:abstractText
Thymidylate kinase catalyzes the phosphorylation of thymidine 5'-monophosphate to thymidine 5'-diphosphate in the pathway of synthesis of dTTP from dTMP. We have purified the enzyme approximately 5000-fold from a plasmid-bearing strain of the yeast Saccharomyces cerevisiae that over produces the activity 6-fold. The protein appears homogeneous by sodium dodecyl sulfate-polyacrylamide gel analysis and has a molecular weight of 25,000. The amino acid composition and the sequence of amino acids on the NH2 terminus have been determined. Our interest in thymidylate kinase stems from the fact that R. A. Sclafani and W. Fangman (personal communication) recently presented genetic evidence that this enzyme is encoded by the CDC8 gene of yeast. In this paper, we show, by several biochemical criteria, that thymidylate kinase is the product of the CDC8 gene. First, extracts of strains bearing six different alleles of cdc8 show no thymidylate kinase activity. Secondly, strains carrying the CDC8 gene on a high-copy-number plasmid produce 6-fold higher levels of the kinase activity than does wild type. Third, the DNA sequence of the CDC8 gene reveals an open reading frame that encodes a protein with the same amino-terminal sequence as purified thymidylate kinase.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11052-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
The CDC8 gene of yeast encodes thymidylate kinase.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't