Linked Life Data

6088488

Source:http://linkedlifedata.com/resource/pubmed/id/6088488

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1984-9-28
pubmed:databankReference
pubmed:abstractText
RNA polymerase III promoters were constructed by cloning chemically synthesized double-stranded analogues of the box A and box B consensus sequences into suitable vectors. In contrast to approaches adopted previously for the analysis of RNA polymerase III promoters, this method has no limitation on the structure and number of variants generated and allows critical sequences in various permutations to be studied. Furthermore, the series of synthetic polymerase III promoters created constitute a collection of point mutation variants and hence provide a powerful tool for the analysis of nucleotides essential for promoter function. The results demonstrate that these two boxes, when separated by 51 base pairs, are sufficient to direct efficient transcription and that substitution of certain nucleotides causes reduced template activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10208-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Construction and functional analysis of a series of synthetic RNA polymerase III promoters.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't