Linked Life Data

6088233

Source:http://linkedlifedata.com/resource/pubmed/id/6088233

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-10-24
pubmed:abstractText
The structure gene encoding CDPdiacylglycerol-inositol 3-phosphatidyltransferase was isolated by complementation in a Saccharomyces cerevisiae mutant after transformation with a comprehensive gene library containing Sau3AI partial restriction fragments of wild-type yeast DNA inserted into the YEp13 shuttle vector. Introduction of the cloned plasmid into the mutant restored both growth of cells and CDPdiacylglycerol-inositol 3-phosphatidyltransferase activity. A subcloning study indicated that the complementing gene was contained within a 1700-base-pair fragment of DNA. Expression of the cloned sequence was independent of its orientation in the vector. CDPdiacylglycerol-inositol 3-phosphatidyltransferase activity in the transformant was eight fold higher than that in the wild-type strain. Although the mutant enzyme had a greatly increased Km for myo-inositol, the enzyme in the transformant had an apparent Km for myo-inositol equal to that of the wild-type enzyme, indicating the presence of the wild-type structure gene on the recombinant plasmid. The elevated level of enzyme activity in the transformant did not lead to an increase in the content of phosphatidylinositol. In contrast, supplementation of the culture medium with increasing concentrations of myo-inositol resulted in an increase in the phosphatidylinositol content. Thus, the availability of myo-inositol is a critical regulatory factor in yeast phosphatidylinositol synthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-6
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Molecular cloning of the gene encoding CDPdiacylglycerol-inositol 3-phosphatidyl transferase in Saccharomyces cerevisiae.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't