Linked Life Data

6087681

Source:http://linkedlifedata.com/resource/pubmed/id/6087681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 2
pubmed:dateCreated
1984-9-17
pubmed:abstractText
Na+/H+ antiporter activity in renal brush border vesicles from control rabbits and rabbits made acidotic with 3 days of NH4Cl gavage was measured using the acridine orange method. Acidotic rabbits exhibited a significantly higher Vmax for antiporter activity (2.80 +/- 0.45 fluorescence units X s-1 X mg protein-1) compared with controls (1.31 +/- 0.13) but the Km for Na+ was unchanged (23.7 +/- 3.5 for acidotic, 19.1 +/- 3.2 mM for controls). When the Vmax for Na+/H+ antiporter activity was considered in relation to the degree of acidosis achieved in the experimental animals, there was a correlation (r = -0.75) between Vmax and plasma total CO2 concentration. Amiloride (100 microM) inhibited Na+/H+ exchange (Na+ = 90 mM) by 59 +/- 7% in both control and acidotic animals, indicating that the observed stimulation in Na+/H+ antiporter activity was not due to increased electrically coupled cation exchange. These findings suggest that the response of the proximal tubule to chronic metabolic acidosis involves an adaptive increase in the Vmax for Na+/H+ antiporter activity in the brush border membrane that is correlated to the degree of acidosis in the animals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
247
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F339-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Increased Vmax for Na+/H+ antiporter activity in proximal tubule brush border vesicles from rabbits with metabolic acidosis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.