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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1984-8-24
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pubmed:databankReference |
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pubmed:abstractText |
A full length N-ras gene has been cloned from both the human fibrosarcoma cell line HT1080 and from normal human DNA. N-ras isolated from HT1080 will efficiently induce morphological transformation of NIH/3T3 cells in a transfection assay, whereas N-ras isolated from normal human DNA has no effect on NIH/3T3 cells. The coding regions of the normal N-ras gene have been sequenced and the predicted amino acid sequence of the N-ras product is very similar to that of the c-Ha-ras1 and c-Ki-ras2 products. By making chimeric molecules between the two cloned genes the activating alteration in the HT1080 N-ras gene has been localised to a single base change that results in an amino acid alteration at position 61 of the p21 N-ras product.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6086315-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6086315-286319,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6086315-7177195
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1321-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6086315-Amino Acid Sequence,
pubmed-meshheading:6086315-Animals,
pubmed-meshheading:6086315-Base Sequence,
pubmed-meshheading:6086315-Cell Line,
pubmed-meshheading:6086315-Cell Transformation, Neoplastic,
pubmed-meshheading:6086315-Cells, Cultured,
pubmed-meshheading:6086315-Chimera,
pubmed-meshheading:6086315-Cloning, Molecular,
pubmed-meshheading:6086315-DNA, Neoplasm,
pubmed-meshheading:6086315-DNA Restriction Enzymes,
pubmed-meshheading:6086315-Fibrosarcoma,
pubmed-meshheading:6086315-Humans,
pubmed-meshheading:6086315-Mice,
pubmed-meshheading:6086315-Mice, Inbred Strains,
pubmed-meshheading:6086315-Oncogenes
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pubmed:year |
1984
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pubmed:articleTitle |
Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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