Linked Life Data

6085020

Source:http://linkedlifedata.com/resource/pubmed/id/6085020

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1985-5-20
pubmed:abstractText
The poly(A)+RNAs produced during DNA puff formation in the salivary gland of R. americana were used to detect the DNA sequences involved in their transcription, using the Southern hybridization and in situ hybridization techniques. DNA prepared from salivary gland after DNA puff regression and carcass were cleaved with EcoRI and hybridized to poly(A)+RNA. After hybridization two major bands corresponding to sizes of 3.0 and 6.0 kb were detected. The hybridization level in the salivary gland DNA was approximately 5-fold that observed with carcass DNA. After in situ hybridization, approximately 10 chromosome regions were labelled. The most highly labelled chromosome regions were C3d and C8e. These regions have been described as DNA puffs that undergo amplification at a specific stage of larval development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0100-879X
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Hybridization of poly(A)+RNA from salivary glands of Rhynchosciara americana to restriction DNA fragments and polytene chromosomes.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't