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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1985-2-25
|
| pubmed:abstractText |
The influence of pH on the reactivity of anti-Chlamydia monoclonal antibodies with genus-, species- and type-specific activity to chlamydial elementary body (EB) antigens was studied by solid-phase enzyme immunoassay. The antibodies bound readily to EB antigen from pH 4 to 9, but had a reduced binding efficiency at more extreme pH conditions. Antibodies although having a wide pH range of optimal binding, bound non-specifically at acidic pH (4-6). This non-specific binding was not seen with polyclonal guinea pig or rabbit anti-Chlamydia antibodies. Thus it seems to be a specific property of mouse immunoglobulins when tested against cell antigen.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
31
|
| pubmed:volume |
75
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
211-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6084029-Antibodies, Monoclonal,
pubmed-meshheading:6084029-Antigen-Antibody Complex,
pubmed-meshheading:6084029-Antigens, Bacterial,
pubmed-meshheading:6084029-Chlamydia,
pubmed-meshheading:6084029-Cross Reactions,
pubmed-meshheading:6084029-Epitopes,
pubmed-meshheading:6084029-Hydrogen-Ion Concentration,
pubmed-meshheading:6084029-Species Specificity
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Effect of pH on reactivity of monoclonal antibodies to Chlamydia.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|