Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1967-5-12
pubmed:abstractText
It is doubtful that the glyoxylate bypass plays a significant role in the accumulation of fumaric acid by fungi, as has been postulated. In high glucose media, which favor fumarate production, isocitrate lyase (threo-D(s) isocitrate glyoxylate lyase), which is the key enzyme of the glyoxylate bypass, is strongly repressed. The specific activity of this enzyme remains low as long as glucose is present in the medium, even though fumarate formation proceeds at a high level. In addition, the activity of isocitrate lyase is inhibited by phosphoenolpyruvate, which would be formed from glucose. Alternatively, evidence is presented that bulk accumulation of fumaric acid under aerobic conditions in high glucose media takes place through a C(3) plus C(1) carbon dioxide fixation. CO(2) fixation was measured by the direct incorporation of NaHC(14)O(3) into fumaric acid, and by demonstrating that the specific radioactivity of fumaric acid formed from uniformly labeled C(14)-glucose was decreased in the presence of nonradioactive carbonate. The extent of decrease in specific radioactivity is in accord with a C(3) plus C(1) CO(2) fixation mechanism.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-13152066, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-13271377, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-13529829, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-13610881, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-13766702, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-14068532, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-14102849, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-15398809, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-16560228, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-16561577, http://linkedlifedata.com/resource/pubmed/commentcorrection/6020565-16588511
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
600-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1967
pubmed:articleTitle
Mechanism of fumaric acid accumulation in Rhizopus nigricans.
pubmed:publicationType
Journal Article