Linked Life Data

597867

Source:http://linkedlifedata.com/resource/pubmed/id/597867

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
1978-3-21
pubmed:abstractText
Nuclei are shown to undergo generalized changes of state during regeneration, as reflected by changes in equilibrium density of sucrose density gradients. The time-course of these phenomena suggests an association with changes occurring after S-phase and mitosis. Such changes are no longer observable in extensively purified nuclei (washed, polyamine-stabilized nuclei), but are apparent only in nuclei with minimal purification, rapidly isolated by centrifugation from liver homogenates. This effect implies that there exists a serious risk of selective loss of some nuclear types during standard purification procedures that use 2.1-2.4 M sucrose cushions. Although these nuclear states may largely reflect the metabolic state of the cells of origin, evidence is presented to suggest that loss and redistribution of macromolecules during handling procedures causes distortion of in vivo properties.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0045-6039
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-72
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Unstable, heterogeneous physical states of nuclear bodies during the post-hepatectomy dedifferentiation-replication-redifferentiation sequence.
pubmed:publicationType
Journal Article