Linked Life Data

597742

Source:http://linkedlifedata.com/resource/pubmed/id/597742

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1978-3-21
pubmed:abstractText
The lining osteoblasts of the rat tibial endosteum were treated for various times (between 1 and 60 min) and increasing doses (3.87-38.7 microgram/ml) with the macrolide fungal metabolite cytochalasin B (CB). Dimethylsulfoxide (DMSO) served as the vehicle for CB and was administered to control tissues. An in vitro incubation system and scanning electron microscopy (SEM) were used to evaluate the effects of CB. The drug initiated cell rounding (contraction), nuclear protrusion, bleb formation, and the subsequent clustering of blebs and microvilli into aggregates. All CB-induced aberrations of the surface structure were readily reversible when tissues were washed with CB-free media. DMSO had no significant effect on cell surface morphology. The reversible structural changes brought about by CB are explained in terms of alterations of the subplasmalemmal cortical microfilament system of osteoblasts. The potential use of CB in evaluating the bone cell cytoskeleton and its relationship to the physiology of the cellular functional membrane (endosteum) of bone is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0008-0594
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-10
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
The effect of cytochalasin B on the endosteal lining cells of mammalian bone. A scanning electron microscopic study.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.