Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1967-8-26
pubmed:abstractText
1. A method in use for the extraction of urinary steroid conjugates has been applied to study the recovery of synthetic steroid monoglucuronides from aqueous solution. 2. In the presence of dissolved ammonium sulphate (50g./100ml.), ether-ethanol (3:1, v/v, 3x0.5vol.) extracted the monoglucuronides of steroids of the C(18), C(19) and C(21) series, quantitatively at values pH2-9. 3. The hydrolysis of the synthetic steroid monoglucuronides by beta-glucuronidase (Patella vulgata) has been examined with reference to the pH value of the medium, enzyme concentration and substrate concentration. 4. The rate of hydrolysis of steroid monoglucuronides was dependent upon steroid structure and upon site of conjugation. 5. The rate of hydrolysis of the monoglucuronides decreased in the order C-3 (phenolic) >C-3beta>C-17beta>C-3alpha.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
631-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1966
pubmed:articleTitle
The extraction and hydrolysis of steroid monoglucuronides.
pubmed:publicationType
Journal Article