Linked Life Data

5943611

Source:http://linkedlifedata.com/resource/pubmed/id/5943611

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1966-10-12
pubmed:abstractText
Using a double-beam stopped-flow apparatus estimations were made of the velocity constant for the initial uptake of oxygen by fully reduced erythrocytes (k'(c)). Mammalian cells were studied with volumes varying from 20 micro(3) (goat) to 90 micro(3) (man), as were bullfrog cells (680 micro(3)). Measurements were made under physiological conditions of pH, P(CO2), and temperature. In man k'(c) was 80 mM(-1) sec(-1) and in other species smaller cells generally had a greater value for k'(c) than did the larger cells. In the goat it was 1.8 times as great as the human value; in the bullfrog it was only one-fifth as great. These differences could not be accounted for by interspecific differences in hemoglobin kinetics. The differences probably represent a true effect of size conferring some biological advantage on the species with the smaller cells. The cell membrane offered resistance to oxygen passage. Using the usual red cell model of an infinite sheet of reduced hemoglobin, membrane permeability appeared to differ among mammals. If, as is likely, the effective cell halfthickness differs among mammals, actual membrane permeability differences may be less. A method for measurement of oxygen saturation of dilute cell suspensions is also described.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1295
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
727-42
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1966
pubmed:articleTitle
The effect of size of red cells on the kinetics of their oxygen uptake.
pubmed:publicationType
Journal Article, In Vitro