Linked Life Data

5821012

Source:http://linkedlifedata.com/resource/pubmed/id/5821012

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1969-11-4
pubmed:abstractText
1. The effects of synthetic fluorocitrate were studied on: (a) the oxidation of citrate and cis-aconitate by rat liver mitochondria; (b) the activity of the aconitate hydratase found in the liver cell sap; (c) the activity of the aconitate hydratase solubilized from liver mitochondria. 2. Fluorocitrate was found to be a potent inhibitor of oxidation of citrate but only a weak inhibitor of oxidation of cis-aconitate: 6.7mum-fluorocitrate (containing 4% of the inhibitory isomer) caused 94% inhibition of the oxidation of citrate (2mm) whereas 1.0mm-fluorocitrate was necessary to provoke the same inhibition when cis-aconitate (2mm) was the substrate. The degree of inhibition varied in relation to the respiratory state of mitochondria when fluorocitrate was added. The inhibition could be partially reversed by cis-aconitate. 3. The aconitate hydratase extracted from the mitochondria was much less inhibited by fluorocitrate than was the mitochondria-bound enzyme, and the aconitate hydratase found in the cell sap was even less sensitive. 0.3mm-Fluorocitrate was required to cause 50% inhibition of the reaction citrate-->cis-aconitate, catalysed by the aconitate hydratase extracted from the mitochondria, and 1.2m-fluorocitrate for the extramitochondrial enzyme. For both enzymes the reaction citrate-->cis-aconitate was 2-3 times more sensitive to fluorocitrate than was the reaction isocitrate-->cis-aconitate. The inhibition was of the competitive type for both reactions.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-13047350, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-13126098, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-13208639, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-13313307, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-13734781, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-13783115, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-14353940, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-14911820, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-14944527, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-15403927, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-15406444, http://linkedlifedata.com/resource/pubmed/commentcorrection/5821012-5766654
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
853-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1969
pubmed:articleTitle
The inhibition by fluorocitrate of rat liver mitochondrial and extramitochondrial aconitate hydratase.
pubmed:publicationType
Journal Article, In Vitro