Linked Life Data

581473

Source:http://linkedlifedata.com/resource/pubmed/id/581473

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1979-2-23
pubmed:abstractText
Discrepancies between calorimetric and fluorescence depolarization monitoring of the pretransition in multilamellar vesicles of synthetic phosphatidylcholines are shown to result primarily from the slow rate of this transition. The depolarization of fluorescence of the membrane-associated dye 1,6-diphenyl-1,3,5-hexatriene was used to determine the temperature of the pretransition for a series of heating and cooling scan rates. These temperatures, when plotted vs. scan rate, extrapolated linearly to the transition temperature at zero-scan rate, Tm = 29.8 +/- 0.8 degrees C. The slopes obtained from these plots yielded characteristic times for the transition of 8 to 30 min. In addition, analysis of temperature-jump experiments, assuming first-order kinetics, gave characteristic times in the range 4--8 min. The data are taken to suggest a most likely value for the pretransition characteristic time of 5 +/- 2 min, with larger values possibly explainable by supercooling effects. Slight differences between the calorimetrically and fluorimetrically determined main transition temperatures appear to result from perturbation of the phosphatidylcholine bilayer by the fluorescent probe.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4475-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Fluorescence and calorimetric studies of phase transitions in phosphatidylcholine multilayers: kinetics of the pretransition.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.