Linked Life Data

5780550

Source:http://linkedlifedata.com/resource/pubmed/id/5780550

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1969-6-5
pubmed:abstractText
1. The aggregation of blood platelets was measured in vitro at different time intervals after the addition of 5-hydroxytryptamine (5-HT) or of reserpine to platelet-rich plasma of untreated rabbits and of rabbits injected with reserpine and 5-HT, i.e. while the platelets were taking up 5-HT or releasing it under the influence of reserpine.2. When 5-HT was added to stirred platelet-rich plasma the platelets aggregated reversibly within 1 min. The velocity of aggregation increased with 5-HT concentrations of 0.1-30 muM and decreased with higher concentrations.3. (-)-Adrenaline, which alone did not produce aggregation, markedly accelerated the aggregation caused by 5-HT. The acceleration was greatest when 5-HT and adrenaline were added simultaneously.4. 5-HT added to the platelet-rich plasma in amounts that exceeded the 5-HT capacity of the platelets progressively diminished the velocity of aggregation produced by 5-HT plus adrenaline until aggregation was completely inhibited. Smaller amounts of 5-HT produced a transient inhibition of aggregation.5. The aggregation of platelets from reserpinized rabbits was inhibited by less 5-HT than the aggregation of platelets from normal rabbits.6. (-)-Adrenaline aggregated platelets of untreated rabbits but not those of reserpinized rabbits or of rabbits injected with 5-HT when reserpine was added in vitro 1-30 min previously.7. Platelets obtained from rabbits treated first with reserpine and subsequently injected with 5-HT were not aggregated by 5-HT plus adrenaline. During incubation in vitro these platelets progressively recovered their aggregability but this recovery was delayed by the monoamine oxidase inhibitor Pargyline.8. Imipramine in concentrations which did not influence platelet aggregation by adenosine diphosphate (ADP) abolished aggregation produced by 5-HT or by 5-HT plus adrenaline.9. The inhibitory effect of adenosine on platelet aggregation was concentration-dependent and similar whether aggregation was produced by ADP, 5-HT or 5-HT plus adrenaline.10. It is proposed that aggregation brought about by 5-HT is connected with its active uptake into the platelets and is caused by ADP formed from ATP during the active uptake of the amine. When 5-HT is no longer actively taken up, it also ceases to cause or to potentiate aggregation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-13320304, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-13665673, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-13673393, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-13871375, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-14056485, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-14230207, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-14347088, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-14421526, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-4379173, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-4870578, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-5645287, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-5664040, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-5780549, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-6034169, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-6055941, http://linkedlifedata.com/resource/pubmed/commentcorrection/5780550-6080058
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:volume
201
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-23
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1969
pubmed:articleTitle
5-Hydroxytryptamine uptake and release in relation to aggregation of rabbit platelets.
pubmed:publicationType
Journal Article