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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1969-1-23
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pubmed:abstractText |
1. Methods for the purification of dog submandibular-gland hyaluronidase from sedimentable and non-sedimentable portions of a homogenate and from the whole homogenate are presented. The method consists of three main steps: removal of mucin by acid precipitation or gel filtration on Sephadex G-200, ammonium sulphate precipitation and CM-cellulose chromatography. By this method specific activities of up to 1.28 and 0.78mumoles of N-acetylglucosamine/min./mg. of protein were obtained for the purified freeze-dried non-sedimentable hyaluronidase and for the sedimentable hyaluronidase respectively. 2. A comparison of some of the properties of the non-sedimentable and the sedimentable hyaluronidase preparation indicated that there was little difference between the two and that they both resembled lysosomal hyaluronidase from rat liver.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-13117829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-13479434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-13886865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-14427329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-14794645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-15390401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-16749142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-4301907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-5640335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-6022841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-6049405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5722688-6067145
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
110
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-25
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:5722688-Animals,
pubmed-meshheading:5722688-Chemical Precipitation,
pubmed-meshheading:5722688-Chromatography,
pubmed-meshheading:5722688-Chromatography, Gel,
pubmed-meshheading:5722688-Dogs,
pubmed-meshheading:5722688-Hyaluronoglucosaminidase,
pubmed-meshheading:5722688-Hydrogen-Ion Concentration,
pubmed-meshheading:5722688-Kinetics,
pubmed-meshheading:5722688-Methods,
pubmed-meshheading:5722688-Submandibular Gland
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pubmed:year |
1968
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pubmed:articleTitle |
Canine submandibular-gland hyaluronidase. Purification and properties.
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pubmed:publicationType |
Journal Article
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