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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1976-8-2
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pubmed:abstractText |
Three types of density gradients - neutral metrizamide, alkaline NaOH-metrizamide and alkaline triethanolamine-metrizamide - were used for studying the distribution of histones between the two DNA strands in alkali-denatured chromatin. It was found possible to avoid both protein redistribution and dissociation by using triethanolamine-metrizamide density gradients at pH 10.5. Under these conditions an alkali-denatured mixture of DNA and chromatin was well separated into the original DNA and DNP. When native or sonicated chromatin was denatured at pH 12.2 and centrifuged in a triethanolamine-metrizamide density gradient at pH 10.5 no peak of free DNA appeared. These results show that both DNA strands remain associated with histone molecules upon alkaline denaturation of chromatin.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-1111096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-1112406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-1122558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-1130676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-1143324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-13293190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-241637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-4524758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-4524789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-4604489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-4688012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-4825889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-4838981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-4838982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-5481244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5708-5821533
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
697-707
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1976
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pubmed:articleTitle |
Distribution of histones in alkali-denatured chromatin studied by isopycnic centrifugation in alkaline metrizamide density gradients.
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pubmed:publicationType |
Journal Article
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