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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1978-7-24
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pubmed:abstractText |
Infectious pancreatic necrosis virus exhibited an interference phenomenon that resulted in the survival of the infected cell with one hit kinetics. The responsible factor was found to co-purify with standard virus through a purification regime that employed two CsCl gradients and a sucrose gradient. This result suggested that a defective interfering (DI) viral particle was involved. It was possible to estimate the number of DI particles by a statistical method using the Poisson distribution that related cell survival to input DI/cell, which indicated that virus samples from dilute passage contained as many DI particles as samples from undiluted passage; this means that multiple undiluted virus passage did not increase the yield of DI particles. In isopycnic CsCl gradient centrifugation, the DI particles were found in a broad band superimposed over the standard virus peak and extending above it, such that the ratio DI/PFU varied from 0.3--20 in different fractions. These centrifugation methods did not completely separate DI particles from standard virus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0304-8608
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
77-89
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:566090-Animals,
pubmed-meshheading:566090-Cell Line,
pubmed-meshheading:566090-Centrifugation, Density Gradient,
pubmed-meshheading:566090-Defective Viruses,
pubmed-meshheading:566090-Reoviridae,
pubmed-meshheading:566090-Salmonidae,
pubmed-meshheading:566090-Viral Interference,
pubmed-meshheading:566090-Virus Replication
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pubmed:year |
1978
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pubmed:articleTitle |
Quantitative analysis of defective interfering particles in infectious pancreatic necrosis virus preparations.
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pubmed:publicationType |
Journal Article
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