Linked Life Data

565826

Source:http://linkedlifedata.com/resource/pubmed/id/565826

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1978-7-26
pubmed:abstractText
The site of recombination of a mink cell focus-inducing strain (Mo-MuLV83) derived from an ecotropic Moloney murine leukemia virus (Mo-MuLV) was mapped by fingerprint analysis of the large RNase T1-resistant oligonucleotides, employing a two-dimensional gel electrophoresis method. Mo-MuLV83, in contrast to the ecotropic Mo-MuLV, demonstrated a broadened host range, i.e., growth not only on mouse cells but also on mink cells, and recombination involved the env gene function. The genomic RNA of these two viruses shared 42 out of a total of 51 to 53 large T1 oligonucleotides (81%) and possessed a similar subunit size of 36S. Most of these T1 oligonucleotides were mapped in their relative order to the 3' polyadenylic acid end of the viral RNA molecules. There were 10 common oligonucleotides immediately next to the 3' termini. A cluster of 7 (in Mo-MuLV83) or 10 (in Mo-MuLV) unique T1 oligonucleotides were mapped next to the common sequences at the 3' end, and they all appeared concomitantly in a polyadenylic acid-containing RNA fraction with a sedimentation coefficient slightly larger than 18S. Therefore, the env gene of Mo-MuLV was situated at a location approximately 2,000 to 4,000 nucleotides from the 3' end of the genomic RNA, and the gene order of Mo-MuLV appeared to be similar to that of the more rigorously determined avian oncornaviruses. cDNA(SFFV) specific for the xenotropic sequences in the spleen focus-forming virus RNA hybridized to the cluster of unique oligonucleotides of Mo-MuLV83 RNA. This suggests that the loci of recombination involve the homologous env gene region of a xenotropic virus.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-1061140, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-1271520, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-170411, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-176365, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-176429, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-184965, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-186781, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-194058, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-200927, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-200928, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-50896, http://linkedlifedata.com/resource/pubmed/commentcorrection/565826-57666
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-83
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Mapping host range-specific oligonucleotides within genomes of the ecotropic and mink cell focus-inducing strains of Moloney murine leukemia virus.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.