Linked Life Data

560868

Source:http://linkedlifedata.com/resource/pubmed/id/560868

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1977-10-20
pubmed:abstractText
Phosphatidylserine has been prepared from phosphatidylcholine by a one-step transphosphatidylation catalyzed by phospholipase D in the presence of L-serine. The resulting mixture of phosphatidylserine and phosphatidic acid is easily and rapidly separated by CM-cellulose column chromatography using step=wise elution with solvents containing increasing percentages of methanol in chloroform. The over-all yield of the procedure is 40-50% depending on the scale of the preparation. CM-Cellulose column chromatography proved to be extremely useful in separating phospholipid mixtures obtained by phosphatidyltransferase reactions of phospholipase D and is also suitable for fractionation of other lipid extracts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
488
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-42
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography.
pubmed:publicationType
Journal Article