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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1981-2-26
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pubmed:abstractText |
Ribosomes and ribosomal proteins from wild-type and three yellow mutants of Chlamydomonas reinhardii were analyzed and compared by two-dimensional gel-electrophoresis. Mixotrophycally grown mutants differ from wild-type cells in lowered chlorophyll content. Analytical ultracentrifugation analysis of cell extracts showed a reduced amount of 70S ribosomes and an increased level of 50S subunits in mutants y-27 and y-28. Two-dimensional gel electrophoresis indicated considerable alterations in the protein composition of 70S ribosomes of the mutants. Two proteins of 70S ribosomes were altered in the y-27 and y-28 mutants. Two proteins were absent from the electrophoretograms of the yellow-76 mutant and seven proteins were present in lowered concentrations. The mutations are inherited in a non-Mendelian manner. The protein alterations in 70S ribosome are most probably localized in the chloroplast DNA.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:author | |
pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
229-39
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:553441-Chlamydomonas,
pubmed-meshheading:553441-Chlorophyll,
pubmed-meshheading:553441-Chloroplasts,
pubmed-meshheading:553441-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:553441-Molecular Weight,
pubmed-meshheading:553441-Mutation,
pubmed-meshheading:553441-Ribosomal Proteins,
pubmed-meshheading:553441-Ribosomes
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pubmed:year |
1979
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pubmed:articleTitle |
Yellow mutations alter chloroplast ribosomal proteins in Chlamydomonas reinhardii.
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pubmed:publicationType |
Journal Article
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