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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1971-11-3
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pubmed:abstractText |
Characteristics of nystatin and amphotericin B action on thin (<100 A) lipid membranes are: (a) micromolar amounts increase membrane conductance from 10(-8) to over 10(-2) Omega(-1) cm(-2); (b) such membranes are (non-ideally) anion selective and discriminate among anions on the basis of size; (c) membrane sterol is required for action; (d) antibiotic presence on both sides of membrane strongly favors action; (e) conductance is proportional to a large power of antibiotic concentration; (f) conductance decreases approximately 10(4) times for a 10 degrees C temperature rise; (g) kinetics of antibiotic action are also very temperature sensitive; (h) ion selectivity is pH independent between 3 and 10, but (i) activity is reversibly lost at high pH; (j) methyl ester derivatives are fully active; N-acetyl and N-succinyl derivatives are inactive; (k) current-voltage characteristic is nonlinear when membrane separates nonidentical salt solutions. These characteristics are contrasted with those of valinomycin. Observations (a)-(g) suggest that aggregates of polyene and sterol from opposite sides of the membrane interact to create aqueous pores; these pores are not static, but break up (melt) and reform continuously. Mechanism of anion selectivity is obscure. Observations (h)-(j) suggest-NH(3) (+) is important for activity; it is probably not responsible for selectivity, particularly since four polyene antibiotics, each containing two-NH(3) (+) groups, induce ideal cation selectivity. Possibly the many hydroxyl groups in nystatin and amphotericin B are responsible for anion selectivity. The effects of polyene antibiotics on thin lipid membranes are consistent with their action on biological membranes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-14212282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-14304893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-14476933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-4230180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5514158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5584619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5641121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5647133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5672005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5673304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5915181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-5967438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-6033739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-6034767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-6065682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5514157-6082492
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1295
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
100-24
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:5514157-Amphotericin B,
pubmed-meshheading:5514157-Cell Membrane Permeability,
pubmed-meshheading:5514157-Chemical Phenomena,
pubmed-meshheading:5514157-Chemistry,
pubmed-meshheading:5514157-Chlorides,
pubmed-meshheading:5514157-Electric Conductivity,
pubmed-meshheading:5514157-Electrophysiology,
pubmed-meshheading:5514157-Hydrogen-Ion Concentration,
pubmed-meshheading:5514157-Lipids,
pubmed-meshheading:5514157-Lithium,
pubmed-meshheading:5514157-Membrane Potentials,
pubmed-meshheading:5514157-Membranes, Artificial,
pubmed-meshheading:5514157-Nystatin,
pubmed-meshheading:5514157-Potassium,
pubmed-meshheading:5514157-Sodium,
pubmed-meshheading:5514157-Temperature
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pubmed:year |
1970
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pubmed:articleTitle |
The ion permeability induced in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B.
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pubmed:publicationType |
Journal Article
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