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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1971-1-28
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pubmed:abstractText |
Capsular antisera against the 72 types of Klebsiella have been prepared in rabbits by using an acetone-dried vaccine. It was shown that encapsulated cells dried with acetone and ground to a fine powder with a mortar and pestle retain their capsules. Antisera obtained from rabbits vaccinated with these vaccines had quellung titers ranging from 1:16 to 1:64. The dried vaccine was stable after storage for over 2 years at room temperature. Encapsulated cultures lyophilized in the presence of 5% sucrose, 5% sodium glutamate, and 5% polyvinylpyrrolidone remained viable and retained their capsules after storage for 10 months at room temperature.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0003-6919
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
416-20
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:5485721-Acetone,
pubmed-meshheading:5485721-Agglutination Tests,
pubmed-meshheading:5485721-Animals,
pubmed-meshheading:5485721-Bacterial Vaccines,
pubmed-meshheading:5485721-Freeze Drying,
pubmed-meshheading:5485721-Immune Sera,
pubmed-meshheading:5485721-Klebsiella,
pubmed-meshheading:5485721-Methods,
pubmed-meshheading:5485721-Preservation, Biological,
pubmed-meshheading:5485721-Rabbits,
pubmed-meshheading:5485721-Temperature,
pubmed-meshheading:5485721-Time Factors,
pubmed-meshheading:5485721-Vaccination
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pubmed:year |
1970
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pubmed:articleTitle |
Use of acetone-dried vaccines for preparing capsular antisera against the Klebsiella group and the lyophilization of Klebsiella cultures.
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pubmed:publicationType |
Journal Article
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