Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1970-12-11
pubmed:abstractText
The enzyme, l-glutamine d-fructose 6-phosphate amidotransferase (EC 2.6.1.16) of Neurospora crassa, which catalyzes the formation of glucosamine 6-phosphate was shown to be subject to feedback inhibition by uridine diphosphate N-acetyl-d-glucosamine (UDP-GlcNAc). The conclusion is based on the following observations. UDP-GlcNAc, the direct precursor of chitin, did not accumulate in the cell even when its utilization for the synthesis of cell wall chitin was interrupted by the antibiotic polyoxin D, a competitive inhibitor of the chitin synthetase (EC 2.4.1.16). Furthermore, the cellular level of UDP-GlcNAc rose in a short period of time when the amidotransferase was bypassed in vivo by the addition of glucosamine to the growing medium of the fungus. The amidotransferase was purified from N. crassa approximately 85-fold. Kinetic studies showed that UDP-GlcNAc was a potent and specific inhibitor of the amidotransferase, and that it did not alter the Michaelis constant for either l-glutamine or d-fructose 6-phosphate, suggesting that the inhibitor binds at a site on the enzyme distinct from the active site.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-13475353, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-13475355, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-13672971, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-13827775, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-14206604, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-14253441, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-14253442, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-14299608, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-14329291, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-14482181, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-4961641, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-5330234, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-5330665, http://linkedlifedata.com/resource/pubmed/commentcorrection/5474877-5353899
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
103
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
588-94
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1970
pubmed:articleTitle
Feedback inhibition of L-glutamine D-fructose 6-phosphate amidotransferase by uridine diphosphate N-acetylglucosamine in Neurospora crassa.
pubmed:publicationType
Journal Article