Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1970-7-24
pubmed:abstractText
1. The Barrett (1967) assay for cathepsin D was slightly modified. 2. The enzyme was purified from liver of man and chicken by a procedure involving autolysis, acetone fractionation, ion-exchange chromatography and isoelectric focusing. 3. Several isoenzymes of cathepsin D were resolved in the isoelectric-focusing step, and three major forms, alpha,beta and gamma, were distinguished for each species. 4. A modified analytical method of isoelectric focusing in polyacrylamide gel indicated a high degree of homogeneity of the purified beta and gamma isoenzymes from each species, and this was supported by their constant high specific activities. 5. Gel filtration of the isoenzymes in a calibrated column of Sephadex G-100 showed that each had a molecular weight of 45000. 6. Human cathepsin D had a pH optimum of 3.5, and that of chicken enzyme was 3.0, haemoglobin being used as substrate. In each species, the three isoenzymes have the same pH-dependence curve. 7. The purified cathepsin D samples showed very little action on acid-denatured albumin.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-14240539, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-14434765, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-14462361, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-14927673, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-4173116, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-4174344, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-4175676, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-4886478, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-4981915, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-4982838, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-5339944, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-5344610, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-5683249, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-5778401, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-5862401, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-5938933, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-5942436, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-6021045, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-6052503, http://linkedlifedata.com/resource/pubmed/commentcorrection/5419752-6058110
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
601-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1970
pubmed:articleTitle
Cathepsin D. Purification of isoenzymes from human and chicken liver.
pubmed:publicationType
Journal Article