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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1970-3-24
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pubmed:abstractText |
The beta-glucosidases (EC 3.2.1.21) of Neurospora crassa were studied with respect to their location in conidia and young mycelia. Aryl-beta-glucosidase of conidia was nearly equally divided between extracellular and bound activity. Bound aryl-beta-glucosidase was almost all available to substrate. An induction procedure was used to maximize both beta-glucosidases in 4 to 6-hr cells. Aryl-beta-glucosidase was entirely bound but still mostly (90%) detectable, whereas cellobiase was mostly internal and cryptic. A freeze-thaw cycle or treatment with phenethyl alcohol or deoxycholic acid made the cellobiase detectable without releasing it from the cell. A 10 to 20% increase in cell-bound aryl-beta-glucosidase could be obtained by this treatment. Dilute HCl (0.1 n) destroyed the patent aryl-beta-glucosidase but not the cryptic aryl-beta-glucosidase or the cryptic cellobiase activity in intact cells. This suggested that most aryl-beta-glucosidase activity was exterior to the cell membrane but still within the mural space. The thermal stability of patent aryl-beta-glucosidase and released cellobiase was found to be higher than in corresponding cell-free extracts. Measurements of K(m) suggested a slightly lower affinity for substrate p-nitrophenyl-beta-d-glucopyranoside by the enzymes in intact cells compared to enzymes in extracts.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-13083617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-13534699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-13725544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-13867668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14089421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14137612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14179673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14201170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14203175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14233912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14233913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14240937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14324993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-14456211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-16562036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-16590440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-4234673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-5225520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-5337048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-5871816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-5927207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5413819-5970513
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
408-17
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1970
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pubmed:articleTitle |
Localization of the beta-glucosidases in Neurospora crassa.
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pubmed:publicationType |
Journal Article
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