Linked Life Data

5409802

Source:http://linkedlifedata.com/resource/pubmed/id/5409802

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1970-2-5
pubmed:abstractText
Washed human platelets are capable of depositing 1-4 as well as probable 1-6 glucosyl linkages onto preexistent glycogen primer. They are also capable of degrading (glycogenolysis) newly synthesized 1-4 as well as probable 1-6 glucosyl linkages. A higher rate of glycogen synthesis was found in platelet suspensions containing lower concentrations of platelets. This was shown to result from decreased glycogen degradation and consequent increased residual glycogen primer in low platelet suspensions. The increased glycogen content of low platelet suspensions was not a result of platelet washing, removal of platelets from plasma, or release of platelet metabolites into the media. The enzyme glycogen synthetase was found to be present at a rate of 5.2 mumoles of uridine diphosphate (UDP) glucose incorporated into glycogen per gram platelets per hour at 37 degrees C. The K(m) for UDP glucose was 6.6 mmoles/liter. At optimum concentration of glucose 6-phosphate, the K(m) was reduced 4.6 fold and V(max) was increased 4.3-fold. Human platelets contain the glyconeogenic pathway. They incorporate pyruvate-(14)C and citrate-(14)C into platelet glycogen and contain an apparent fructose-1,6-diphosphatase. The apparent fructose-1,6-diphosphatase was activated by adenosine monophosphate (AMP) and adenosine diphosphate (ADP), inhibited by adenosine triphosphate (ATP), and shown to be rate limiting for glyconeogenesis at physiologic concentration of adenine nucleotide.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-13781408, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-14035641, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-14109181, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-14275118, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-4286494, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-4291394, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-4868314, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-4980135, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-5337434, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-5675431, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-5771188, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-5780848, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-5863087, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-6023775, http://linkedlifedata.com/resource/pubmed/commentcorrection/5409802-6038304
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
140-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1970
pubmed:articleTitle
Glycogenesis and glyconeogenesis in human platelets. Incorporation of glucose, pyruvate, and citrate into platelet glycogen; glycogen synthetase and fructose-1,6-diphosphatase activity.
pubmed:publicationType
Journal Article, In Vitro