pubmed-article:534529 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:534529 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
pubmed-article:534529 | lifeskim:mentions | umls-concept:C0040711 | lld:lifeskim |
pubmed-article:534529 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
pubmed-article:534529 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
pubmed-article:534529 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
pubmed-article:534529 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:534529 | pubmed:dateCreated | 1980-3-24 | lld:pubmed |
pubmed-article:534529 | pubmed:abstractText | Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of purification achieved is described. Cell-free protein-synthesizing systems from unstimulated lymphocytes translate added lymphocyte mRNA with lower efficiency than do comparable systems from phytohaemagglutinin-activated lymphocytes, whatever the source of the mRNA used. | lld:pubmed |
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pubmed-article:534529 | pubmed:language | eng | lld:pubmed |
pubmed-article:534529 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:534529 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:534529 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:534529 | pubmed:month | Nov | lld:pubmed |
pubmed-article:534529 | pubmed:issn | 0264-6021 | lld:pubmed |
pubmed-article:534529 | pubmed:author | pubmed-author:KayJ EJE | lld:pubmed |
pubmed-article:534529 | pubmed:author | pubmed-author:WallaceD MDM | lld:pubmed |
pubmed-article:534529 | pubmed:author | pubmed-author:JagusRR | lld:pubmed |
pubmed-article:534529 | pubmed:author | pubmed-author:BenzieC RCR | lld:pubmed |
pubmed-article:534529 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:534529 | pubmed:day | 15 | lld:pubmed |
pubmed-article:534529 | pubmed:volume | 184 | lld:pubmed |
pubmed-article:534529 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:534529 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:534529 | pubmed:pagination | 277-82 | lld:pubmed |
pubmed-article:534529 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:534529 | pubmed:year | 1979 | lld:pubmed |
pubmed-article:534529 | pubmed:articleTitle | Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes. | lld:pubmed |
pubmed-article:534529 | pubmed:publicationType | Journal Article | lld:pubmed |
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