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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1980-3-24
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pubmed:abstractText |
Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of purification achieved is described. Cell-free protein-synthesizing systems from unstimulated lymphocytes translate added lymphocyte mRNA with lower efficiency than do comparable systems from phytohaemagglutinin-activated lymphocytes, whatever the source of the mRNA used.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-1001766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-1008846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-1060069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-1125228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-1132427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-1180897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-1268259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-165487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-169893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-200446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-321129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4181589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4340753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4525168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-459918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4732074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4824348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4835728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4835729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4842657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-4896715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-5045304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-5505329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-6035921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-668908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-782287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/534529-786155
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
277-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:534529-Animals,
pubmed-meshheading:534529-Cell-Free System,
pubmed-meshheading:534529-Cytoplasm,
pubmed-meshheading:534529-Lymphocyte Activation,
pubmed-meshheading:534529-Lymphocytes,
pubmed-meshheading:534529-Peptide Chain Initiation, Translational,
pubmed-meshheading:534529-Phytohemagglutinins,
pubmed-meshheading:534529-Poly A,
pubmed-meshheading:534529-Protein Biosynthesis,
pubmed-meshheading:534529-RNA, Messenger,
pubmed-meshheading:534529-Swine
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pubmed:year |
1979
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pubmed:articleTitle |
Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.
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pubmed:publicationType |
Journal Article
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