Linked Life Data

5321479

Source:http://linkedlifedata.com/resource/pubmed/id/5321479

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1966-2-19
pubmed:abstractText
Curtiss, Roy, III (Oak Ridge National Laboratory, Oak Ridge, Tenn.), Leigh J. Charamella, Claire M. Berg, and Paula E. Harris. Kinetic and genetic analyses of d-cycloserine inhibition and resistance in Escherichia coli. J. Bacteriol. 90:1238-1250.1965.-Wild-type cells of Escherichia coli growing at 37 C in mineral salts-glucose medium with vigorous aeration were lysed at maximal exponential rates by 10(-4) to 10(-2)md-cycloserine. At concentrations above 2 x 10(-2)m, d-cycloserine was bacteriostatic. Low levels of d-cycloserine (10(-5)m) and pencillin G (10 units per ml) interacted synergistically to cause a rapid exponential rate of lysis. Spontaneous mutations to d-cycloserine resistance occurred in discrete steps at frequencies of 10(-6) to 10(-7) for each step. First-, second-, and third-step d-cycloserine-resistant mutants were lysed at maximal exponential rates by d-cycloserine concentrations of 10(-3), 3 x 10(-3), and 5 x 10(-3)m, respectively. d-Alanine, l-alanine, and dl-alanyl-dl-alanine reversed d-cycloserine-induced lysis, in that order of effectiveness. On the basis of these observations, a d-cycloserine-enrichment cycling technique was developed for isolation of auxotrophic mutants. d-Cycloserine at 2 x 10(-3)m was as efficient as penicillin G (1,000 units per ml) for mutant enrichment in E. coli and should be useful for isolation of mutants in penicillin-resistant microorganisms. Bacterial conjugation experiments indicated that all three mutations conferring d-cycloserine resistance were linked to the met(1) locus. Transduction experiments showed that the mutation conferring first-step resistance was at least 0.5 min away from the mutations conferring second- and third-step resistance. The latter two mutations possibly occurred in the same gene, since they were sometimes carried in the same transducing phage. Studies on expression of d-cycloserine resistance indicated that these mutations were neither dominant nor recessive to each other nor to the d-cycloserine-sensitivity allele. Each allelic state exerted its influence on the phenotype independently of the others. These results are discussed in terms of the known inhibition of alanine racemase and d-alanyl-d-alanine synthetase by d-cycloserine.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-13267987, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-13485077, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-13795638, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-13796629, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-13851300, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-13872629, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14042952, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14047211, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14221875, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14255660, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14255678, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14362438, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14446135, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14467046, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14479178, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-14927572, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-15436457, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-16561951, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-16562043, http://linkedlifedata.com/resource/pubmed/commentcorrection/5321479-17247995
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1238-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1965
pubmed:articleTitle
Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli.
pubmed:publicationType
Journal Article, In Vitro