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Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3688
|
pubmed:dateCreated |
1965-11-17
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pubmed:abstractText |
The survival of Escherichia coli B/r WP2 (tryptophan-requiring) from ultraviolet irradiation when suspended in 0.067M phosphate buffer (pH 7) has been studied over the temperature range 22 degrees to -269 degrees C. In unfrozen suspensions there was no appreciable change in sensitivity between 22 degrees and -10 degrees C. The sensitivity in the presence of ice progressively increased by a factor of 7 when the temperature was lowered to -79 degrees C. Between -79 degrees and -196 degrees C the sensitivity decreased to less than four times the sensitivity at 22 degrees C and was not appreciably different at -269 degrees C. Evidence from experiments with bacteriophage T1 and E. coli WP2 HCR(-) (a strain unable to excise thymine dimers) indicates that a new, qualitatively different lesion, less amenable to repair, may replace the thymine dimer in E. coli irradiated at -79 degrees C.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0036-8075
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
3
|
pubmed:volume |
149
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1103-5
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pubmed:dateRevised |
2010-10-22
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pubmed:meshHeading |
pubmed-meshheading:5318093-Cold Temperature,
pubmed-meshheading:5318093-Coliphages,
pubmed-meshheading:5318093-Culture Media,
pubmed-meshheading:5318093-Escherichia coli,
pubmed-meshheading:5318093-Freezing,
pubmed-meshheading:5318093-Radiation Genetics,
pubmed-meshheading:5318093-Thymine,
pubmed-meshheading:5318093-Tryptophan,
pubmed-meshheading:5318093-Ultraviolet Rays
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pubmed:year |
1965
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pubmed:articleTitle |
Ultraviolet damage to bacteria and bacteriophage at low temperatures.
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pubmed:publicationType |
Journal Article,
In Vitro
|