Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1976-1-2
pubmed:abstractText
Conventional methods (i.e. gradient centrifugation) for the purification of oncornaviruses are usually not effective in complete removal of nonviral proteins. Such contaminants often prove to be a nuisance in subsequent immunological or biochemical studies. Hyperimmune sera prepared from these viruses must be absorbed to assure specificity; cell-derived proteins can be shown to interfere with studies of virus structural proteins, nucleic acids, or viral enzymes. Herein is described a method for removal of most of these contaminants. Viruses are diluted in a high concentration of NaCl to achieve a final concentration of 15%, incubated for 30 min, sedimented, and resuspended in buffer. This procedure results in reductions of up to 48% of the protein without affecting particle count. Immunological, biochemical, and biological properties are not adversely affected. Of the proteins removed, fetal calf serum components and a ribonuclease (presumably cell-derived) were identified. This technique differs significantly from other high-salt methods in that the virus is not precipitated from suspension. It is believed that absorbed proteins are desorbed and left in solution (or suspension) as the virus is sedimented by centrifugation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4098102, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4118589, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4122326, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4197065, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4201521, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4297122, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4330470, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4621398, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4921121, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-4925356, http://linkedlifedata.com/resource/pubmed/commentcorrection/51858-6057678
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
253-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
New method for the removal of extraneous proteins from purified oncornaviruses.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.