Linked Life Data

510087

Source:http://linkedlifedata.com/resource/pubmed/id/510087

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-2-28
pubmed:abstractText
During the early cleavage divisions in some Ascarids, parts of the chromosomes are eliminated from the somatic blastomeres ("chromatin diminution", Boveri, 1887) while the chromosomes in the germ line cells maintain their integrity. To characterize the germ line and soma genome, DNA was isolated from gametes and embryonic somatic cells of two Ascarid species, Parascaris equorum var. univalens and Ascaris suum. It was shown that the germ line limited DNAs of these species have the same density and almost identical reassociation kinetics: in CsCl the predominant component of the germ line limited DNA of P. equorum and A. suum has the buoyant density of 1.697 g/cm3, while soma DNA of both species bands at 1.700 g/cm3. In P. equorum there is a small additional germ line limited satellite DNA component with the density of 1.690 g/cm3, identical to that of mitochondrial DNA of both organisms. Comparison of the reassociation kinetics of germ line and soma DNA demonstrates for both species that the eliminated DNA sequences are highly repetitive. In contrast to these similarities between the germ line limited DNAs of P. equorum and A. suum the analysis of their base composition revealed differences (40% guanine plus cytosine in P. equorum and 36% in A. suum). The only very fast reassociating DNA sequences which we could isolate from soma DNA was demonstrated to be foldback DNA. The reassociation kinetics of total A. suum soma DNA was investigated by hydroxylapatite chromatography. Least squares analysis of the data revealed about 10% of intermediate repetitive DNA sequences. Their interspersion between single copy DNA sequences was analyzed by comparing the reassociation kinetics of DNA fragments 0.35 and 7.2 kilobases long. Thus the DNA sequence arrangement of Ascaris does not follow the short period interspersion pattern observed in most organism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-5915
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
355-71
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Satellite DNA properties of the germ line limited DNA and the organization of the somatic genomes in the nematodes Ascaris suum and Parascaris equorum.
pubmed:publicationType
Journal Article