Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1972-11-29
pubmed:abstractText
A method is described for the isolation of mitochondrial malate dehydrogenase from either the whole tissue homogenate or from the microsomal fraction of rat liver. The procedure involves the treatment of the tissue extract with detergent followed by gel filtration and chromatography on Amberlite CG-50 and DEAE-cellulose. The resulting enzyme was homogeneous by the criterion of gel electrophoresis. Incubation of the microsomal fraction from rat liver under the usual conditions for protein synthesis in the presence of [(3)H]leucine resulted in the incorporation of (3)H into the mitochondrial malate dehydrogenase when purified as described. The results are taken to indicate that the mitochondrial enzyme is synthesized by the cytoplasmic ribosomes. Possible ways in which the cytoplasmic and mitochondrial forms of malate dehydrogenase reach their final locations in the cell are discussed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-13984966, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-14063281, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-14240539, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-16742570, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-4166536, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-4296320, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-4304512, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-4309867, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-4315931, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-4389820, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-4920823, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-5459577, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-5583987, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-5657877, http://linkedlifedata.com/resource/pubmed/commentcorrection/5075231-6038170
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
126
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-5
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1972
pubmed:articleTitle
Studies on the biosynthesis of mitochondrial malate dehydrogenase and the location of its synthesis in the liver cell of the rat.
pubmed:publicationType
Journal Article, In Vitro