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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1972-7-3
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pubmed:abstractText |
In a defined medium with added ethionine plus low methionine, phenylalanine, tryptophan, tyrosine, adenine, and additional methionine reversed inhibition of the enteric yeast Candida slooffii by ethionine. Isoleucine and 7-methylguanine restored half-maximal growth. Choline but not triethylcholine inhibited C. slooffii. 6-Mercaptopurine reversed ethionine inhibition and also synergistic inhibition by ethionine plus choline. Protection against ethionine by adenine plus aromatics was also evident with log-phase cells in the absence of methionine. Incorporation of ethionine-ethyl-1-(14)C by resting cells was partially inhibited by aromatic amino acids and methionine. Ethionine depressed incorporation of (3)H-phenylalanine but not of (3)H-adenine. Ethionine-resistant mutants were isolated which incorporated ethionine efficiently and degraded it to yet unidentified substances not including 5'-ethylthioadenosine. Ethionine-sensitive cells accumulated more S-adenosylethionine (SAE) than resistant mutants. Adenine was a good precursor of SAE. Radioactivity from ethionine-ethyl-1-(14)C was recovered from cell fractions of ethionine-sensitive cells with the following distribution: cold trichloroacetic acid-soluble > hot trichloroacetic acid-insoluble > lipids > deoxyribonucleic acid > ribonucleic acid. Total radioactivity recovered from ethionine-sensitive cells was twice as much as that from ethionine-resistant mutants.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-13271380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-13575393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-13780063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-13981612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14149680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14153769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14172634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14197889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14225078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14273646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14291586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14774400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14907676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14917666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-14955514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-4866912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-5339953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-5474888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-5786780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-5867300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-5877967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-5969263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-6005629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5022172-6057806
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/6-Mercaptopurine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Ethionine,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/Purines,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
110
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
643-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:5022172-6-Mercaptopurine,
pubmed-meshheading:5022172-Adenine,
pubmed-meshheading:5022172-Amino Acids,
pubmed-meshheading:5022172-Candida,
pubmed-meshheading:5022172-Carbon Isotopes,
pubmed-meshheading:5022172-Cell Fractionation,
pubmed-meshheading:5022172-Choline,
pubmed-meshheading:5022172-Chromatography, Paper,
pubmed-meshheading:5022172-Culture Media,
pubmed-meshheading:5022172-Depression, Chemical,
pubmed-meshheading:5022172-Drug Resistance, Microbial,
pubmed-meshheading:5022172-Ethionine,
pubmed-meshheading:5022172-Genetics, Microbial,
pubmed-meshheading:5022172-Methionine,
pubmed-meshheading:5022172-Mutation,
pubmed-meshheading:5022172-Phenylalanine,
pubmed-meshheading:5022172-Purines,
pubmed-meshheading:5022172-Pyrimidines,
pubmed-meshheading:5022172-Stereoisomerism,
pubmed-meshheading:5022172-Tritium
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pubmed:year |
1972
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pubmed:articleTitle |
Metabolism of ethionine in ethionine-sensitive and ethionine-resistant cells of the enteric yeast Candida slooffii.
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pubmed:publicationType |
Journal Article
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