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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1972-6-28
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pubmed:abstractText |
1. The cation composition of single barnacle muscle fibres following damage by axial insertion of a microsyringe has been measured. The Na and Ca contents of these fibres were raised.2. Electronmicroscopic studies of fibres following insertion of a microsyringe indicated that the damage done resulted in tubular obstruction of the T-system.3. Fibres loaded with radiosodium by micro-injection showed that the Na(*) efflux declined exponentially with time, but that in most fibres the slope ratio of d/dt ln [Na(*)](1) to d/dt (ln d[Na(*)](i)/dt) was less than unity. Injections of distilled water deep in the fibre failed to influence the course of the Na(*) efflux.4. K removal reduced the Na efflux by 47%. However, a few fibres displayed very little K-dependence.5. When measured in fibres already soaked in a K-free medium for long periods the sodium efflux consisted of a brief rapid phase, followed by a slow phase of Na loss.6. In the presence of 30 mM-K, there was little or no rise in the Na efflux. Raising the external K to 50 or 100 mM caused a marked rise in the Na efflux. Raising the external K to 30 mM in the absence of external Ca(2+) led to a rise in the Na efflux. A high K solution always caused shortening of these fibres.7. Internal application of 1 M or 1 mM-CaCl(2) often caused a significant rise in the Na efflux.8. Internal application of 2.5 or 5 M saline caused a prompt and large fall in the Na efflux. In the presence of high K saline-loaded fibres failed to contract.9. Internal application of 0.5 M-ATP stimulated the Na efflux. A larger effect was not observed in fibres pre-treated with 2 M-MgCl(2). Internal application of 0.5 M-ArP was without effect.10. The results indicate that the barnacle fibre is a suitable preparation for the study of Na fluxes by means of the micro-injection technique. They also indicate that the mechanism regulating the Na efflux is not quite the same as that found in squid axon or frog muscle.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-13320357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-14079672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-14109756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-14212145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-14284780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-14478315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-14825236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5112238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5139495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5229806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5246543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5415616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5541585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5574858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5651768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5660179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5943616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-5972170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-6050106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5020983-6064140
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
221
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
389-414
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:5020983-Adenosine Triphosphate,
pubmed-meshheading:5020983-Animals,
pubmed-meshheading:5020983-Biological Transport, Active,
pubmed-meshheading:5020983-Calcium,
pubmed-meshheading:5020983-Calcium Chloride,
pubmed-meshheading:5020983-Injections,
pubmed-meshheading:5020983-Membrane Potentials,
pubmed-meshheading:5020983-Methods,
pubmed-meshheading:5020983-Microscopy, Electron,
pubmed-meshheading:5020983-Muscles,
pubmed-meshheading:5020983-Myofibrils,
pubmed-meshheading:5020983-Phosphates,
pubmed-meshheading:5020983-Potassium,
pubmed-meshheading:5020983-Sodium,
pubmed-meshheading:5020983-Sodium Isotopes,
pubmed-meshheading:5020983-Thoracica,
pubmed-meshheading:5020983-Time Factors
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pubmed:year |
1972
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pubmed:articleTitle |
An investigation of sodium transport in barnacle muscle fibres by means of the microsyringe technique.
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pubmed:publicationType |
Journal Article
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