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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1975-10-10
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pubmed:abstractText |
During fixation of single muscles fibers with glutaraldehyde, the volume of the fiber shrinks 20%, recovers in rinse and osmium tetroxide to near normal volume and shrinks 20% again when staining with uranyl acetate. This suggest that osmotic properties of membranes may not have been completely lost during fixation, post-fixation and en bloc staining. Dehydration in ethanol and propylene oxide produces a further 10% shrinkage in volume. Infiltration and embedding with Epon causes an additional 15% change in volume. This gives a total shrinkage in volume of 45% which is nearly twice that of the apparent shrinkage in the volume of the myosin lattice as determined by electron microscopy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0040-8166
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
383-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:49940-Acetates,
pubmed-meshheading:49940-Animals,
pubmed-meshheading:49940-Anura,
pubmed-meshheading:49940-Ethanol,
pubmed-meshheading:49940-Ethers, Cyclic,
pubmed-meshheading:49940-Glutaral,
pubmed-meshheading:49940-Histological Techniques,
pubmed-meshheading:49940-Microscopy, Electron,
pubmed-meshheading:49940-Muscles,
pubmed-meshheading:49940-Myosins,
pubmed-meshheading:49940-Osmium,
pubmed-meshheading:49940-Propane,
pubmed-meshheading:49940-Specimen Handling,
pubmed-meshheading:49940-Staining and Labeling,
pubmed-meshheading:49940-Uranium
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pubmed:year |
1975
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pubmed:articleTitle |
Size changes in single muscle fibers during fixation and embedding.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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