Linked Life Data

497269

Source:http://linkedlifedata.com/resource/pubmed/id/497269

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1980-1-19
pubmed:abstractText
It was shown that metyrapone, the inhibitor of arylhydrocarbonhydroxylase, taken at concentrations equimolar to that of cytochrome P-450 non-competitively inhibits the hydroxylation of 3,4-benzpyrene in the liver microsomes of inbred mice of the C57BL/6 and AKR strains. In a given "mutual depletion inhibition system" the concentration of the catalytically active centres of microsomal cytochrome (Ecac), their turnover number (TNcac) and "true" dissociation constant of the enzyme-inhibitor complex were determined in the control and 3-methylcholanthrenetreated mice of both strains. The increased rate of 3,4-benzpyrene hydroxylation in the liver of 3-methylcholanthrene-induced "sensitive" C57BL/6 mice is determined by the increase of Ecac (and, in a lesser degree, of TNcac) per molecule of cytochrome P-448. In the liver microsomes of "induction-resistant" AKR mice an injection of 3-methylcholanthrene results in a slight increase of Ecac and a simultaneous decrease of TNcac. It was assumed that contrary to the present-day concepts, an aberrant microsomal hemoprotein with a genetically determined low molecular activity is synthesized in mice of "resistant" AKR strain.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0320-9725
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1172-83
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
[Use of inhibition by metyrapone in a "mutual depletion system" for evaluation of active centres of various arylhydrocarbon hydroxylases].
pubmed:publicationType
Journal Article, Comparative Study, English Abstract