| pubmed-article:4960898 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:4960898 | lifeskim:mentions | umls-concept:C0004595 | lld:lifeskim |
| pubmed-article:4960898 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:4960898 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:4960898 | lifeskim:mentions | umls-concept:C1160185 | lld:lifeskim |
| pubmed-article:4960898 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:4960898 | pubmed:dateCreated | 1967-7-22 | lld:pubmed |
| pubmed-article:4960898 | pubmed:abstractText | The effects on transformation and transduction of an ultraviolet sensitivity (uvr(-)) and two ultraviolet sensitivity-recombination deficiency (rec-1(-) and rec-2(-)) mutations in isogenic strains of Bacillus subtilis were investigated. Transformation frequency in the rec-1(-) and rec-2(-) strains was reduced to approximately 5 and 25%, respectively, of the parental strains. Normal kinetics of deoxyribonucleic acid dose response in transformation were found for the rec-1(+) and rec-2(-) strains. Biphasic curves were obtained with the rec-1(-) strains. Transduction frequency with bacteriophage SP-10 decreased parallel to transformation frequency in the rec-1(-) and rec-2(-) strains. This result suggests that transformation and SP-10 transduction share a common mechanism for genetic recombination. It also indicates that the reduction in transformation frequency of these strains was not due to altered competence. Transduction frequency with bacteriophage PBS-1 or 3NT, on the contrary, was not diminished in rec-1(-) strains. This frequency was reduced in rec-2(-) strains but not as severely as that of transformation or SP-10 transduction. Several hypotheses to interpret these differences are presented. Recombination frequency between linked markers was reduced more than 50% in transformation by the presence of the rec-1(-) mutation. Linkage was unaffected in the rec-2(-) strains. Neither the rec-1(-) nor the rec-2(-) mutation had an effect on linkage in PBS-1 or 3NT transduction. The uvr(-) strains were transformed at a frequency equal to or greater than that of the parental strains. These strains were transduced by all bacteriophage systems at frequencies about twofold higher than those of parental strains. | lld:pubmed |
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| pubmed-article:4960898 | pubmed:language | eng | lld:pubmed |
| pubmed-article:4960898 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:4960898 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:4960898 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:4960898 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:4960898 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:4960898 | pubmed:author | pubmed-author:Anagnostopoul... | lld:pubmed |
| pubmed-article:4960898 | pubmed:author | pubmed-author:HochJ AJA | lld:pubmed |
| pubmed-article:4960898 | pubmed:author | pubmed-author:BaratMM | lld:pubmed |
| pubmed-article:4960898 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:4960898 | pubmed:volume | 93 | lld:pubmed |
| pubmed-article:4960898 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:4960898 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:4960898 | pubmed:pagination | 1925-37 | lld:pubmed |
| pubmed-article:4960898 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:4960898 | pubmed:meshHeading | pubmed-meshheading:4960898-... | lld:pubmed |
| pubmed-article:4960898 | pubmed:year | 1967 | lld:pubmed |
| pubmed-article:4960898 | pubmed:articleTitle | Transformation and transduction in recombination-defective mutants of Bacillus subtilis. | lld:pubmed |
| pubmed-article:4960898 | pubmed:publicationType | Journal Article | lld:pubmed |
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