Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1967-7-22
|
| pubmed:abstractText |
The effects on transformation and transduction of an ultraviolet sensitivity (uvr(-)) and two ultraviolet sensitivity-recombination deficiency (rec-1(-) and rec-2(-)) mutations in isogenic strains of Bacillus subtilis were investigated. Transformation frequency in the rec-1(-) and rec-2(-) strains was reduced to approximately 5 and 25%, respectively, of the parental strains. Normal kinetics of deoxyribonucleic acid dose response in transformation were found for the rec-1(+) and rec-2(-) strains. Biphasic curves were obtained with the rec-1(-) strains. Transduction frequency with bacteriophage SP-10 decreased parallel to transformation frequency in the rec-1(-) and rec-2(-) strains. This result suggests that transformation and SP-10 transduction share a common mechanism for genetic recombination. It also indicates that the reduction in transformation frequency of these strains was not due to altered competence. Transduction frequency with bacteriophage PBS-1 or 3NT, on the contrary, was not diminished in rec-1(-) strains. This frequency was reduced in rec-2(-) strains but not as severely as that of transformation or SP-10 transduction. Several hypotheses to interpret these differences are presented. Recombination frequency between linked markers was reduced more than 50% in transformation by the presence of the rec-1(-) mutation. Linkage was unaffected in the rec-2(-) strains. Neither the rec-1(-) nor the rec-2(-) mutation had an effect on linkage in PBS-1 or 3NT transduction. The uvr(-) strains were transformed at a frequency equal to or greater than that of the parental strains. These strains were transduced by all bacteriophage systems at frequencies about twofold higher than those of parental strains.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-13293190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-13683086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-14002700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-14240402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-14294081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-14329448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-14345178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-16561900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-16590310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-4955535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-4955994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-4956864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-4956865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-4958213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-4961652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-4961717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-5335128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-5335129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-5339652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4960898-5340183
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0021-9193
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
93
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1925-37
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1967
|
| pubmed:articleTitle |
Transformation and transduction in recombination-defective mutants of Bacillus subtilis.
|
| pubmed:publicationType |
Journal Article
|