GENERIC 4957028

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0004595, umls-concept:C0012854, umls-concept:C0162544, umls-concept:C0678594, umls-concept:C1444754, umls-concept:C1706536
pubmed:issue	5
pubmed:dateCreated	1966-8-25
pubmed:abstractText	Anderson, D. L. (University of Minnesota, Minneapolis), D. D. Hickman, and B. E. Reilly. Structure of Bacillus subtilis bacteriophage phi29 and the length of phi29 deoxyribonucleic acid. J. Bacteriol. 91:2081-2089. 1966-Bacillus subtilis bacteriophage phi29 were negatively stained with phosphotungstic acid. The head of phi29 has a hexagonal outline with a flattened base, and is about 315 A wide and 415 A in length. The virus has an intricate tail about 325 A in length. Twelve spindle-shaped appendages are attached to the lower of two collars which comprise the proximal portion of the tail. The distal 130 A of the tail axis has a diameter of about 60 A and is larger in diameter than the axis of the upper portion of the tail. Comparison of electron microscopic counts of phi29 with plaque-forming units indicated that about 50% of the microscopic entities were infective. Phenol-extracted phi29 deoxyribonucleic acid (DNA) molecules were prepared for electron microscopy by the cytochrome c film technique of Kleinschmidt et al. Measurement of contour lengths of DNA molecules from three preparations gave skewed distributions of lengths with observed modal class values ranging from 5.7 to 5.9 mu. Assuming that phi29 DNA is a double helix in the B form, the corresponding molecular weights would be 10.9 x 10(6) to 11.3 x 10(6) daltons. The largest DNA molecules would have a volume of 1.9 x 10(7) A(3) which is about 25% greater than the estimated 1.4 x 10(7) A(3) internal volume of the phage head.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-13804200, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-13959257, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-14033431, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-14148437, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-14193626, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-14257342, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-14267270, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-14273661, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-14297210, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-4956049, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-5322728, http://linkedlifedata.com/resource/pubmed/commentcorrection/4957028-5859045
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2985120R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral
pubmed:status	MEDLINE
pubmed:month	May
pubmed:issn	0021-9193
pubmed:author	pubmed-author:AndersonD LDL, pubmed-author:HickmanD DDD, pubmed-author:ReillyB EBE
pubmed:issnType	Print
pubmed:volume	91
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	2081-9
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:4957028-Bacillus subtilis, pubmed-meshheading:4957028-Bacteriophages, pubmed-meshheading:4957028-DNA, Viral, pubmed-meshheading:4957028-Microscopy, Electron, pubmed-meshheading:4957028-Molecular Weight
pubmed:year	1966
pubmed:articleTitle	Structure of Bacillus subtilis bacteriophage phi 29 and the length of phi 29 deoxyribonucleic acid.
pubmed:publicationType	Journal Article, In Vitro