rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1970-11-6
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pubmed:abstractText |
Nutritionally induced filamentous cell forms of Escherichia coli B were examined for their morphological and biochemical lesions. The filamentous forms showed no significant alteration in total DNA concentration, RNA synthesis, ability to form beta-galactosidase in response to isopropylthiogalactoside, or insensitivity to actinomycin D as compared to the normal cell form. The filamentous cells showed a marked decrease in the ability to incorporate N-acetylglucosamine-UL-(14)C into a phenol-soluble glycoprotein fraction relative to the normal cell form or relative to strain E-26 of E. coli grown in the filament-inducing medium. The filaments yielded an envelope-specific phenol-soluble protein fraction markedly reduced in or lacking three proteins as determined by acrylamide gel electrophoresis. Amino acid analysis, and chemical and enzymatic treatments of the envelope-specific phenol-soluble proteins showed striking differences between the fractions obtained from normal and filamentous cells. Electron microscope studies of divalent cation-induced aggregates of the envelope proteins showed different aggregation patterns dependent upon the cell form yielding the protein fraction.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13192056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13208257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13353526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13525667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13549505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13631748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13654246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13671378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-13986422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-14067096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-14187392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-14187398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-15390401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-4290799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-4868364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-4869940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-4872728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-4878430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-4959044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-4969951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-5216369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-5332408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-5333623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-5337850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-5646489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-5650391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-5965351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-6057709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4918213-6064040
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosidases,
http://linkedlifedata.com/resource/pubmed/chemical/Glucosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9525
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
493-508
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:4918213-Amino Acids,
pubmed-meshheading:4918213-Carbohydrate Metabolism,
pubmed-meshheading:4918213-Carbon Isotopes,
pubmed-meshheading:4918213-Cell Membrane,
pubmed-meshheading:4918213-Culture Media,
pubmed-meshheading:4918213-DNA, Bacterial,
pubmed-meshheading:4918213-Electrophoresis, Disc,
pubmed-meshheading:4918213-Escherichia coli,
pubmed-meshheading:4918213-Galactosidases,
pubmed-meshheading:4918213-Glucosamine,
pubmed-meshheading:4918213-Glycoproteins,
pubmed-meshheading:4918213-Microscopy, Electron,
pubmed-meshheading:4918213-Phospholipids,
pubmed-meshheading:4918213-Proteins,
pubmed-meshheading:4918213-RNA, Bacterial,
pubmed-meshheading:4918213-Uridine
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pubmed:year |
1970
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pubmed:articleTitle |
Membrane modifications in nutritionally induced filamentous Escherichia coli B.
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pubmed:publicationType |
Journal Article
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