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rdf:type |
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lifeskim:mentions |
umls-concept:C0005953,
umls-concept:C0007634,
umls-concept:C0018593,
umls-concept:C0024204,
umls-concept:C0037993,
umls-concept:C0061472,
umls-concept:C0085979,
umls-concept:C0301869,
umls-concept:C0348011,
umls-concept:C0596383,
umls-concept:C1517600,
umls-concept:C1705822
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pubmed:issue |
5
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pubmed:dateCreated |
1969-12-16
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pubmed:abstractText |
Hartley guinea pigs genetically unresponsive to hapten-PLL (poly-L-lysine) conjugates were lethally irradiated and given allogeneic bone marrow from Hartley responder animals. Many of the animals died of graft versus host disease before their response to 2,4-dinitrophenyl-PLL (DNP-PLL) could be measured. The immune response of the surviving recipient animals was evaluated by anti-DNP antibody production, development of delayed hypersensitivity to DNP-poly-L-lysine, as well as by lymph node cell stimulation in vitro by this antigen. 12 of 14 recipient animals thus treated made an immune response as measured by 2 of the 3 parameters. Strain 13 guinea pigs, genetically unable to respond immunologically to DNP-PLL and to DNP-GL (2,4-dinitrophenyl-L-glutamic acid L-lysine copolymer) were lethally irradiated and given bone marrow from (2 x 13) F(1) responder animals or strain 13 bone marrow and (2 x 13) F(1) lymph node and spleen cells. A high proportion of the animals survived this procedure; no evidence of graft versus host disease was observed. Three of three strain 13 animals irradiated and, given strain 13 bone marrow and (2 x 13) F(1) lymph node and spleen, and then immunized with DNP-PL, made a specific immune response. 7 of 10 irradiated strain 13 animals given strain 13 bone marrow and (2 x 13) F(1) lymph node and spleen made an immune response to DNP-GL. However, only one of six irradiated strain 13 animals made a vigorous immune response to DNP-GL after reconstitution with (2 x 13) F(1) bone marrow alone. The ability to transfer the immune response to PLL antigens from responder to nonresponder animals demonstrates unequivocally that the defect in the non-responder animals is immunological rather than due to some other type of non-immunological mechanism. The bone marrow contains all the immunological cells necessary for the expression of the PLL gene. However, the finding that (2 x 13) F(1) lymph node and spleen cells were more effective than (2 x 13)F(1) bone marrow cell populations (known to be a rich source of monocyte precursors) suggests that the cells in which the PLL gene function is expressed may be lymphocytes rather than monocytes and macrophages.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-14030666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-14237210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-14295560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-14941986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-4178352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-4880001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-4965466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-4979775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5333748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5648473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5668023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5681653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5691986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5839284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5849233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5865661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-5938816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-6055758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4899853-6062006
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1107-22
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:4899853-Animals,
pubmed-meshheading:4899853-Antibody Formation,
pubmed-meshheading:4899853-Bone Marrow,
pubmed-meshheading:4899853-Bone Marrow Cells,
pubmed-meshheading:4899853-Bone Marrow Transplantation,
pubmed-meshheading:4899853-Glutamates,
pubmed-meshheading:4899853-Guinea Pigs,
pubmed-meshheading:4899853-Haptens,
pubmed-meshheading:4899853-Lymph Nodes,
pubmed-meshheading:4899853-Lymphocytes,
pubmed-meshheading:4899853-Lysine,
pubmed-meshheading:4899853-Nitrophenols,
pubmed-meshheading:4899853-Peptides,
pubmed-meshheading:4899853-Precipitin Tests,
pubmed-meshheading:4899853-Species Specificity,
pubmed-meshheading:4899853-Spleen,
pubmed-meshheading:4899853-Transplantation, Homologous
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pubmed:year |
1969
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pubmed:articleTitle |
Transfer of responsiveness to hapten conjugates of poly-L-lysine and of a copolymer of L-glutamic acid and L-lysine to lethally irradiated nonresponder guinea pigs by bone marrow or lymph node and spleen cells from responder guinea pigs.
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pubmed:publicationType |
Journal Article
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