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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1968-4-10
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pubmed:abstractText |
1. Growth of Escherichia coli on glucosamine results in an induction of glucosamine 6-phosphate deaminase [2-amino-2-deoxy-d-glucose 6-phosphate ketol-isomerase (deaminating), EC 5.3.1.10] and a repression of glucosamine 6-phosphate synthetase (l-glutamine-d-fructose 6-phosphate aminotransferase, EC 2.6.1.16); glucose abolishes these control effects. 2. Growth of E. coli on N-acetylglucosamine results in an induction of N-acetylglucosamine 6-phosphate deacetylase and glucosamine 6-phosphate deaminase, and in a repression of glucosamine 6-phosphate synthetase; glucose diminishes these control effects. 3. The synthesis of amino sugar kinases (EC 2.7.1.8 and 2.7.1.9) is unaffected by growth on amino sugars. 4. Glucosamine 6-phosphate synthetase is inhibited by glucosamine 6-phosphate. 5. Mutants of E. coli that are unable to grow on N-acetylglucosamine have been isolated, and lack either N-acetylglucosamine 6-phosphate deacetylase (deacetylaseless) or glucosamine 6-phosphate deaminase (deaminaseless). Deacetylaseless mutants can grow on glucosamine but deaminaseless mutants cannot. 6. After growth on glucose, deacetylaseless mutants have a repressed glucosamine 6-phosphate synthetase and a super-induced glucosamine 6-phosphate deaminase; this may be related to an intracellular accumulation of acetylamino sugar that also occurs under these conditions. In one mutant the acetylamino sugar was shown to be partly as N-acetylglucosamine 6-phosphate. Deaminaseless mutants have no abnormal control effects after growth on glucose. 7. Addition of N-acetylglucosamine or glucosamine to cultures of a deaminaseless mutant caused inhibition of growth. Addition of N-acetylglucosamine to cultures of a deacetylaseless mutant caused lysis, and secondary mutants were isolated that did not lyse; most of these secondary mutants had lost glucosamine 6-phosphate deaminase and an uptake mechanism for N-acetylglucosamine. 8. Similar amounts of (14)C were incorporated from [1-(14)C]-glucosamine by cells of mutants and wild-type growing on broth. Cells of wild-type and a deaminaseless mutant incorporated (14)C from N-acetyl[1-(14)C]glucosamine more efficiently than from N[1-(14)C]-acetylglucosamine, incorporation from the latter being further decreased by acetate; cells of a deacetylaseless mutant showed a poor incorporation of both types of labelled N-acetylglucosamine.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-13363455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-13426195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-13428742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-13549465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-13702505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-13879763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14034827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14206604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14224387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14342730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14343123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14343124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14370918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14416360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14468226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-14775715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-15436457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-16748273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-4861885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-4862174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-5340309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4866432-5845836
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
847-58
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4866432-Bacteriolysis,
pubmed-meshheading:4866432-Carbon Isotopes,
pubmed-meshheading:4866432-Cell Wall,
pubmed-meshheading:4866432-Chromatography, Ion Exchange,
pubmed-meshheading:4866432-Chromatography, Paper,
pubmed-meshheading:4866432-Culture Media,
pubmed-meshheading:4866432-Enzyme Induction,
pubmed-meshheading:4866432-Enzyme Repression,
pubmed-meshheading:4866432-Escherichia coli,
pubmed-meshheading:4866432-Glucosamine,
pubmed-meshheading:4866432-Glucose,
pubmed-meshheading:4866432-Growth,
pubmed-meshheading:4866432-Isomerases,
pubmed-meshheading:4866432-Mutation,
pubmed-meshheading:4866432-Phosphotransferases,
pubmed-meshheading:4866432-Spectrum Analysis,
pubmed-meshheading:4866432-Transaminases,
pubmed-meshheading:4866432-Ultraviolet Rays
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pubmed:year |
1968
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pubmed:articleTitle |
Control of amino sugar metabolism in Escherichia coli and isolation of mutants unable to degrade amino sugars.
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pubmed:publicationType |
Journal Article
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