|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1974-6-25
|
|
pubmed:abstractText |
KB cells productively infected with human adenovirus type 2 contain an alkalistable class of viral DNA sedimenting in a broad zone between 50 and 90S as compared to 34S for virion DNA. This type of DNA is characterized as viral by DNA-DNA hybridization. It is extremely sensitive to shear fragmentation. Extensive control experiments demonstrate that the fast-sedimenting viral DNA is not due to artifactual drag of viral DNA mechanically trapped in cellular DNA or to association of viral DNA with protein or RNA. Furthermore, the fast-sedimenting DNA is found after infection with multiplicities between 1 and 1,000 PFU/cell and from 6 to 8 h postinfection until very late in infection (24 h). Analysis in dye-buoyant density gradients eliminates the possibility that the fast-sedimenting viral DNA represents supercoiled circular molecules. Upon equilibrium centrifugation in alkaline CsCl density gradients, the fast-sedimenting viral DNA bands in a density stratum intermediate between that of cellular and viral DNA. In contrast, the 34S virion DNA isolated and treated in the same manner as the fast-sedimenting DNA cobands with viral marker DNA. After ultrasonic treatment of the fast-sedimenting viral DNA, it shifts to the density positions of viral DNA and to a lesser extent to that of cellular DNA. The evidence presented here demonstrates that the 50 to 90S viral DNA represents adenovirus DNA covalently integrated into cell DNA.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-13130792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-13675766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-13997382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-14262469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4139804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4299943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4308095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4336565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4342050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4345508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4507213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4627331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4684581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4745628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4776968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-4778808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5021309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5050656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5062681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5105670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5125575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5132696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5157354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5248820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5339143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5477340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5530012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5816953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4824714-6051854
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
13
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
975-92
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:4824714-Adenoviridae,
pubmed-meshheading:4824714-Carbon Radioisotopes,
pubmed-meshheading:4824714-Carcinoma,
pubmed-meshheading:4824714-Cell Line,
pubmed-meshheading:4824714-Centrifugation, Density Gradient,
pubmed-meshheading:4824714-Centrifugation, Zonal,
pubmed-meshheading:4824714-DNA, Neoplasm,
pubmed-meshheading:4824714-DNA, Viral,
pubmed-meshheading:4824714-Formates,
pubmed-meshheading:4824714-Mouth Neoplasms,
pubmed-meshheading:4824714-Nucleic Acid Hybridization,
pubmed-meshheading:4824714-Thymidine,
pubmed-meshheading:4824714-Time Factors,
pubmed-meshheading:4824714-Tritium
|
|
pubmed:year |
1974
|
|
pubmed:articleTitle |
Intracellular forms of adenovirus DNA. 3. Integration of the DNA of adenovirus type 2 into host DNA in productively infected cells.
|
|
pubmed:publicationType |
Journal Article
|
