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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1975-7-18
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pubmed:abstractText |
Normal mouse spleen cells were fractionated in dishes coated with thin layers of DNP-gelatin or NIP-gelatin, which were insoluble at 4 degrees C. Highly viable cells were recovered from the dishes by melting the gel at 37 degrees C. NIP3- gelatin layers bound approximately 0.1% and DNP4-gelatin layers 0.5% of normal spleen cells. Increasing numbers of low affinity cells were bound with increasing DNP density of the adsorbent. The binding to insoluble DNP-gelatin was hapten-specific since it was inhibited by DNP-lysine, soluble DNP-gelatin or DNP-BSA but not by soluble gelatin or bovine serum albumin (BSA). It was also inhibited by a polyvalent rabbit antimouse Ig. DNP-gelatin was detected on the surface of cells recovered from DNP-gelatin-coated dishes by 125-I-labeled anti-DNP Ig. The cell surface bound DNP-gelatin could be removed by treatment with collagenase. Collagenase treatment did not detectably affect cell viability or surface receptors. More than 90% of DNP-gelatin binding cells were labeled with a polyvalent 125-I-labeled antimouse Ig before or after collagenase treatment under conditions known to label B lymphocytes. Furthermore, the specific antigen-binding capacity of the purified cell populations could be demonstrated after treatment with collagenase. Purified DNP4-gelatin binding cells contained more than 100 times as many DNP-RFC than unfractionated cells. The enrichment of NIP-RFC in the cell population recovered from NIP3 gelatin-coated dishes was more than 200-fold.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-14812273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4104294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4110525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4114858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4124982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4137202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4137510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4139227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4540327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4540455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4568186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4582160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4591178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4611686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4912957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4913207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4927599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4934503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-4942707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-5101790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-5289374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-5782770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/47891-5939478
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1004-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:47891-Adsorption,
pubmed-meshheading:47891-Animals,
pubmed-meshheading:47891-Autoradiography,
pubmed-meshheading:47891-Binding Sites,
pubmed-meshheading:47891-Catalysis,
pubmed-meshheading:47891-Cell Separation,
pubmed-meshheading:47891-Dinitrophenols,
pubmed-meshheading:47891-Epitopes,
pubmed-meshheading:47891-Haptens,
pubmed-meshheading:47891-Immune Adherence Reaction,
pubmed-meshheading:47891-Immunoglobulins,
pubmed-meshheading:47891-Iodine Radioisotopes,
pubmed-meshheading:47891-Lymphocytes,
pubmed-meshheading:47891-Mice,
pubmed-meshheading:47891-Mice, Inbred CBA,
pubmed-meshheading:47891-Rabbits,
pubmed-meshheading:47891-Serum Albumin, Bovine,
pubmed-meshheading:47891-Sheep,
pubmed-meshheading:47891-Spleen
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pubmed:year |
1975
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pubmed:articleTitle |
Separation of antigen-specific lymphocytes. I. Enrichment of antigen-binding cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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