rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1974-3-28
|
pubmed:abstractText |
1. The intraperitoneal injection of N-methyl-N-nitrosourea (100mg/kg) caused a partial inhibition of protein synthesis in several organs of the rat, the maximum effect occurring after 2-3h. 2. In the liver the inhibition of protein synthesis was paralleled by a marked disaggregation of polyribosomes and an increase in ribosome monomers and ribosomal subunits. No significant breakdown of polyribosomes was found in adult rat brains although N-methyl-N-nitrosourea inhibited cerebral and hepatic protein synthesis to a similar extent. In weanling rats N-methyl-N-nitrosourea caused a shift in the cerebral polyribosome profile similar to but less marked than that in rat liver. 3. Reaction of polyribosomal RNA with N-[(14)C]methyl-N-nitrosourea in vitro did not lead to a disaggregation of polyribosomes although the amounts of 7-methylguanine produced were up to twenty times higher than those found after administration of sublethal doses in vivo. 4. It was concluded that changes in the polyribosome profile induced by N-methyl-N-nitrosourea may reflect the mechanism of inhibition of protein synthesis rather than being a direct consequence of the methylation of polyribosomal mRNA.
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-14075106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-14240664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-14324825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4230610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4256414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4643764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4673570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4698302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4862936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4898286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-4969994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5041565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5120304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5144850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5163160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5394503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5552389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5555565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5584005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5680613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5722690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5722691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5768434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-5787640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4774397-6068285
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0264-6021
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
136
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
303-9
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:4774397-Animals,
pubmed-meshheading:4774397-Brain,
pubmed-meshheading:4774397-Carbon Radioisotopes,
pubmed-meshheading:4774397-Depression, Chemical,
pubmed-meshheading:4774397-Female,
pubmed-meshheading:4774397-Guanine,
pubmed-meshheading:4774397-Leucine,
pubmed-meshheading:4774397-Liver,
pubmed-meshheading:4774397-Mitochondria, Liver,
pubmed-meshheading:4774397-Nerve Tissue Proteins,
pubmed-meshheading:4774397-Nitrosourea Compounds,
pubmed-meshheading:4774397-Polyribosomes,
pubmed-meshheading:4774397-Protein Biosynthesis,
pubmed-meshheading:4774397-Rats,
pubmed-meshheading:4774397-Ribonucleases,
pubmed-meshheading:4774397-Ribosomes
|
pubmed:year |
1973
|
pubmed:articleTitle |
Inhibition of protein synthesis by N-methyl-N-nitrosourea in vivo.
|
pubmed:publicationType |
Journal Article
|