Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1974-1-4
pubmed:abstractText
The requirement for Zn(++) in DNA replication by phytohemagglutinin-stimulated human lymphocytes was studied. When 6 microM o-phenanthroline, a chelator with a high affinity for Zn(++), is added to cultures of stimulated lymphocytes a nearly complete inhibition of thymidine incorporation results within a few hours. In contrast, the incorporation of uridine is only slightly reduced and the incorporation of leucine is unaffected. m-Phenanthroline, a nonchelating analogue, does not alter the rate of thymidine incorporation even when present in 10-fold greater amounts than o-phenanthroline. The inhibition of thymidine incorporation by o-phenanthroline could be entirely reversed by the addition of Zn(++) to the cultures, or could be prevented by the prior addition of either Zn(++) or Ni(++). All other divalent cations tested were incapable of reversing the o-phenanthroline inhibition of thymidine incorporation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-14088078, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-14427849, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-4119222, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-4335070, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-4552485, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-4632167, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-4940372, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-5246548, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-5542689, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-5556427, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-5765781, http://linkedlifedata.com/resource/pubmed/commentcorrection/4747918-5862227
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
594-601
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1973
pubmed:articleTitle
Zinc requirement for DNA replication in stimulated human lymphocytes.
pubmed:publicationType
Journal Article