Linked Life Data

4737291

Source:http://linkedlifedata.com/resource/pubmed/id/4737291

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1973-10-5
pubmed:abstractText
Several esters of 4-methylumbelliferone and 2-naphthol were synthesized and examined as possible spectrofluorimetric titrants for bovine alpha-chymotrypsin, trypsin, thrombin, Factor Xa and for subtilisin Novo. 4-Methylumbelliferyl p-guanidinobenzoate hydrochloride (MUGB) is a satisfactory titrant for alpha- and beta-trypsin, thrombin and Factor Xa and 4-methylumbelliferyl p-(NNN-trimethylammonium)cinnamate (MUTMAC) is a good titrant for alpha-chymotrypsin. The amount of enzyme used for spectrofluorimetric titration is 0.02-3.00nmol and the amount of 4-methylumbelliferone liberated is independent of the concentration of titrant and stoicheiometrically equal to the amount of enzyme used. Results obtained with MUGB and MUTMAC have been checked by spectrophotometric titration with p'-nitrophenyl p-guanidinobenzoate hydrochloride and p-nitrophenyl N(2)-acetyl-N(1)-benzylcarbazate respectively. p-Nitrophenyl N(2)-acetyl-N(1)-(9-anthrylmethyl)carbazate has been synthesized; it did not react with alpha-chymotrypsin. A satisfactory spectrofluorimetric titrant for subtilisin Novo was not discovered.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-13731648, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-16496527, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-4108500, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-4239491, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5360043, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5460940, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5494855, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5640157, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5642613, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5682313, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5689288, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5690109, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5792567, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5972864, http://linkedlifedata.com/resource/pubmed/commentcorrection/4737291-5980876
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-17
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1973
pubmed:articleTitle
Determination of the operational molarity of solutions of bovine alpha-chymotrypsin, trypsin, thrombin and factor Xa by spectrofluorimetric titration.
pubmed:publicationType
Journal Article